lepr  (R&D Systems)

Journal: Frontiers in Nutrition

Article Title: Astragaloside IV modulates oxidative stress and osteoimmune–Wnt signaling in ovariectomized rats: an integrated study of RNA sequencing, molecular docking, and experimental validation

doi: 10.3389/fnut.2026.1785452

Figure Lengend Snippet: Molecular docking models of Astragaloside IV (AS-IV) binding to the core targets. Representative binding poses of AS-IV (shown in stick representation) within the predicted binding pockets of the key target proteins. The calculated binding free energy (kcal/mol) for each complex is indicated. (A) Il22ra2 (−10.2 kcal/mol), (B) Ptgs2 (−9.5 kcal/mol), (C) Wnt1 (−9.3 kcal/mol), (D) Igfbp2 (−8.4 kcal/mol), (E) GZMB (−8.2 kcal/mol), (F) Lep (−7.8 kcal/mol), (G) Gfap (−7.8 kcal/mol), (H) Wnt10a (−7.7 kcal/mol). These results demonstrate stable binding interactions between the therapeutic compound AS-IV and all eight core targets identified from the PPI network and WGCNA, with Il22ra2 and Ptgs2 showing the strongest binding affinity.

Article Snippet: Primary antibodies diluted in antibody diluent were applied and incubated at 4 °C overnight; the primary antibodies were as follows: PTGS2 (ABclonal, A3560, rabbit, 1:200), IGFBP2 (Proteintech, 66,644-1-IG, mouse, 1:200), WNT1 (ABclonal, A2475, rabbit, 1:200), LEP (bioss, bs-0409R, rabbit, 1:200), GFAP (Proteintech, 60,190-1-IG, mouse, 1:1000), β -catenin (huilanbio, ABB3523, rabbit, 1:200), and NF-κB p65 (Cell Signaling Technology, 8,242, rabbit, 1:200), with EDTA-based retrieval used for all targets.

Techniques: Binding Assay

Journal: Frontiers in Nutrition

Article Title: Astragaloside IV modulates oxidative stress and osteoimmune–Wnt signaling in ovariectomized rats: an integrated study of RNA sequencing, molecular docking, and experimental validation

doi: 10.3389/fnut.2026.1785452

Figure Lengend Snippet: Multiplex immunofluorescence imaging and semi-quantification of target proteins in femoral trabecular bone. Representative multiplex immunofluorescence images showing DAPI (nuclei), target protein staining, and merged overlays in the femoral trabecular region from Sham, OVX, and OVX-AS-IV-M treated rats. Panels show staining for (A) NF-κB p65, (B) Wnt1, (C) Gfap, (D) β-catenin, (E) Igfbp2, (F) Lep, and (G) Ptgs2. Scale bar, 50 μm. The corresponding semi-quantification of mean fluorescence intensity (MFI) for each marker is presented to the right of each image panel, normalized to the Sham group (set to 1). Data are presented as mean ± SD with individual data points overlaid. Statistical comparisons were performed using one-way ANOVA followed by Dunnett’s multiple-comparisons test. * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: Primary antibodies diluted in antibody diluent were applied and incubated at 4 °C overnight; the primary antibodies were as follows: PTGS2 (ABclonal, A3560, rabbit, 1:200), IGFBP2 (Proteintech, 66,644-1-IG, mouse, 1:200), WNT1 (ABclonal, A2475, rabbit, 1:200), LEP (bioss, bs-0409R, rabbit, 1:200), GFAP (Proteintech, 60,190-1-IG, mouse, 1:1000), β -catenin (huilanbio, ABB3523, rabbit, 1:200), and NF-κB p65 (Cell Signaling Technology, 8,242, rabbit, 1:200), with EDTA-based retrieval used for all targets.

Techniques: Multiplex Assay, Immunofluorescence, Imaging, Staining, Fluorescence, Marker

Journal: Aesthetic Plastic Surgery

Article Title: A Rat Model Investigation of Enhanced Facial Rejuvenation via PCL Microsphere-Induced Superior Collagen Neogenesis in the Supraperiosteal Plane

doi: 10.1007/s00266-025-05503-6

Figure Lengend Snippet: Effect of implanted fillers on the recruitment of PSCs and ingrowth of blood vessels following implantation in the periosteum of rat skull for a duration of 1 month. Scale bars, 100 μm. A Representative immunostainings of LepR+ PSCs, Emcn+ vessels, and OPN+ mature osteoblast in HA and PCL fillers. B , C Quantification of the relative Emcn+ vessel area in implants ( B ) and peri-implants ( C ). D Quantification of the relative LepR+ area in implants. Data are means ± SD. * P < 0.05, and *** P < 0.005 (unpaired two-tailed Student’s t test)

Article Snippet: Mouse Leptin Receptor (LepR) biotinylated antibody (BAF497) was sourced from R&D systems (Minneapolis, MN, USA).

Techniques: Two Tailed Test